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Assessment of genetic diversity in EMS-induced porang (Amorphophallus muelleri Blume) revealed by RAPD markers
Haritzah, Rendha Maulana
; Radita, Novia Fitri
; Ramadanti, Isma Salsabila
; Azzahro, Yusri Despa
; Wahyudi, Didik
; Resmisari, Ruri Siti
; Suyono
Increasing genetic diversity of porang through mutation is urgently needed since porang has low genetic diveristy. Therefore, this study aims to increase the genetic diversity of porang using ethyl methanesulfonate (EMS) and analyze the genetic diversity of porang using RAPD markers. Porang explants were subjected to in vitro EMS induction at various concentrations (control, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and evaluated after 35 days. DNA amplification was carried out using 20 RAPD primers. Ge...
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Evidence-based non-residential waste analysis to support 3R strategies and food recovery hierarchy: a case study in Solok Selatan
The amount of waste generation that increases without being accompanied by good waste management will cause pollution and decrease the aesthetic value of the environment. Non-residential waste originating from non-residential activities is also one of the contributors to waste entering the landfill. This study aims to analyze non-residential waste generation and composition as an evidence-based reference for each source in implementing the 3R concept and the Food Recovery Hierarchy (FRH). The nu...
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Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain
Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control...
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Utilization of cassava peel waste (Manihot esculenta) as substrate in the production of cellulase enzymes by Aspergillus niger
Cellulase enzymes is an enzyme that can hydrolyze cellulose into smaller sugar components. Cellulase enzymes are produced due to high demand, with 29.17% in the animal feed industry, 14.67% in the textile industry, and 26.17% in the paper industry. One method of producing cellulase enzymes is by utilizing the cellulolytic microorganism Aspergillus niger. This study aims to optimize cellulase enzyme production by Aspergillus niger through liquid fermentation on cassava peel substrate (Manihot esc...
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Synergistic cultures for resilient mercury bioremediation in ASGM leveraging microbial interactions for sustainable pollutant removal
The widespread use of mercury (Hg) in artisanal and small-scale gold mining (ASGM) necessitates effective bioremediation strategies. This study evaluated the mercury reduction capabilities of two bacterial strains, Bacillus subtilis and Pseudomonas aeruginosa, using real-world ASGM liquid waste. In a seven-day laboratory-scale experiment, we assessed the performance of single and mixed bacterial cultures under varying pH conditions. Our results show that B. subtilis was particularly effective, a...
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Potential of butterfly pea (Clitoria ternatea) nanoherbal on spermatozoa quality in hyperglycemic rats
Hyperglycemia is a condition characterized by elevated blood glucose levels that induce free radical formation, leading to reduced spermatozoa quality. Butterfly pea (Clitoria ternatea) contains antioxidant compounds and is formulated as a nanoherbal to enhance bioavailability. This study aimed to evaluate the potential of butterfly pea nanoherbal in improving spermatozoa quality in hyperglycemic rats. This experimental study employed a post-test only control group design using 25 male Wistar ra...
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Integrating field inventory and in silico phylogenetic analysis of pteridophytes at Gandul Mount, Indonesia
Pteridophytes are vascular plants that are considered the most primitive plants because they only use spores to reproduce. Several major fern families exhibit distinctive morphological and ecological characteristics that support their taxonomic recognition. Pteridophytes represent an important component of tropical biodiversity, yet their diversity and evolutionary relationships in karst ecosystems remain poorly documented. This study aimed to integrate field inventory and in silico phylogenetic...
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Diversity of plankton species in different ecosystems on Beras Basah Island, Bontang, East Kalimantan
Imam Fadila, Muhammad
; Anjasmara, Aang Setyawan
; Rizal, Daisy Rahma
; Rusdi, Rismawaty
; Reynalta, Ricko
Beras Basah Island, located in the coastal waters of Bontang City, East Kalimantan, is a marine conservation area characterized by diverse coastal ecosystems, including marine waters, coral reefs, and seagrass beds. These ecosystems support various aquatic organisms, including plankton communities that play a key role in marine food webs. This study aimed to examine the composition, abundance, and diversity of plankton across three different ecosystems around Beras Basah Island. Sampling was con...
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Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches
Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. an...
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PENGARUH PERSEPSI NILAI LAYANAN DAN KEPUASAN TERHADAP LOYALITAS PELANGGAN DI BANK PERKREDITAN RAKYAT
Probisnis
Vol 19
, No 1
(2026)
Penelitian ini bertujuan untuk menguji pengaruh persepsi nilai layanan dan kepuasan pelanggan terhadap loyalitas nasabah BPR. Penelitian ini mengambil obyek penelitian pada Bank Perkreditan Rakyat yang berada di wilayah Banyumas Raya. Teknik pengambilan sampel menggunakan purposive sampling melalui kuesioner yang bersifat tertutup. Ukuran sampel penelitian adalah 200 responden. Teknik analisis yang dipergunakan adalah SEM-AMOS. Temuan hasil penelitian menunjukkan pengaruh positif persepsi nila...
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