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Morphological and genetic identifications of Spirulina based on 16S rRNA gene
Spirulina is a microalga with spiral or linear trichome shapes that has potential in industrial and food sectors due to its rich nutritional content. Spirulina can live in various aquatic habitats with different environmental conditions. Differences in cultivation environments can affect the morphological characteristics of Arthrospira due to phenotypic plasticity, making morphology-based identification prone to errors. Therefore, molecular identification using the 16S rRNA gene for phylogenetic...
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Assessment of genetic diversity in EMS-induced porang (Amorphophallus muelleri Blume) revealed by RAPD markers
Haritzah, Rendha Maulana
; Radita, Novia Fitri
; Ramadanti, Isma Salsabila
; Azzahro, Yusri Despa
; Wahyudi, Didik
; Resmisari, Ruri Siti
; Suyono
Increasing genetic diversity of porang through mutation is urgently needed since porang has low genetic diveristy. Therefore, this study aims to increase the genetic diversity of porang using ethyl methanesulfonate (EMS) and analyze the genetic diversity of porang using RAPD markers. Porang explants were subjected to in vitro EMS induction at various concentrations (control, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and evaluated after 35 days. DNA amplification was carried out using 20 RAPD primers. Ge...
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Pharmacological prediction of Marchantia polymorpha: GC-MS and molecular docking approaches
Marchantia polymorpha is the liverworts that can be found abundantly in the Mount Pasang Jember area. The metabolite profiling of M. polymorpha has not yet been conducted. The method used in this study was untargeted metabolite profiling using GC-MS, and the results of the metabolite compound profiling were analyzed in silico using bioinformatics-based, namely PASS Online, SwisADME, and Molecular Docking using PyRx 8.0 with AutodockVina. The aims of docking with this protein was adjusted to th...
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Utilization of cassava peel waste (Manihot esculenta) as substrate in the production of cellulase enzymes by Aspergillus niger
Cellulase enzymes is an enzyme that can hydrolyze cellulose into smaller sugar components. Cellulase enzymes are produced due to high demand, with 29.17% in the animal feed industry, 14.67% in the textile industry, and 26.17% in the paper industry. One method of producing cellulase enzymes is by utilizing the cellulolytic microorganism Aspergillus niger. This study aims to optimize cellulase enzyme production by Aspergillus niger through liquid fermentation on cassava peel substrate (Manihot esc...
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Synergistic cultures for resilient mercury bioremediation in ASGM leveraging microbial interactions for sustainable pollutant removal
The widespread use of mercury (Hg) in artisanal and small-scale gold mining (ASGM) necessitates effective bioremediation strategies. This study evaluated the mercury reduction capabilities of two bacterial strains, Bacillus subtilis and Pseudomonas aeruginosa, using real-world ASGM liquid waste. In a seven-day laboratory-scale experiment, we assessed the performance of single and mixed bacterial cultures under varying pH conditions. Our results show that B. subtilis was particularly effective, a...
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Isolation and screening of halotolerant protease-producing bacteria from Lampung fermented shrimp paste
Protease are class of degradative enzymes that hydrolyze proteins, breaking its peptide bond, into smaller peptides and amino acids. Microbial proteases have gained increasing attention due to its productivity and utilization in various industries. Proteolytic bacteria derived from fermented shrimp paste from Lampung have great potential to meet this need. However, studies on these bacteria are still limited, so further research is needed to explore and optimally utilize their potential. This st...
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Mangrove Biodiversity for Coastal Resilience and Sustainability: A Dynamic Case Study from Indonesia
Martuti, Nana Kariada Tri
; Jabbar, Abdul
; Irsadi, Andin
; Sidiq, Wahid Akhsin Budi Nur
; Melati, Inaya Sari
; Eldin Khair, Amar Sharaf
; Mutiatari, Dhita Pracisca
Mangrove degradation and socio-economic vulnerability in the Kendal coast require an integrated approach. The study used ecological surveys (mangrove vegetation analysis, avifauna) and socio-economic (n=186 households). Integrated Coastal Management (ICM) analysis and model were developed using a dynamic approach, encompassing problem identification, conceptual model formulation, and validation preparation. 14 mangrove species (H' index = 1.58–1.80) and 61 bird species (H' = 3.50) were found. Co...
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Comparative Evaluation of Parameter-Efficient Fine-Tuning Strategies for Continual Image Classification
Catastrophic forgetting remains a major challenge in continual transfer learning, where performance on earlier tasks degrades after sequential adaptation. While full fine-tuning updates all parameters and achieves strong performance on new tasks, it is computationally expensive and prone to forgetting. This study compares parameter-efficient fine-tuning (PEFT) methods—adapters, additive learning, side-tuning, LoRA, and zero-initialized layers—against full fine-tuning on CIFAR-100 using a two-sta...
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Exploring the Satisfaction–Revenue Nexus: A Mixed-Methods Analysis of RevPAR Performance in Southeast Asian Three-Star Hotels
This research aims to explore the satisfaction–revenue nexus by examining the effect of tourist satisfaction on Revenue Per Available Room (RevPAR) performance in three-star hotels across Southeast Asia. Grounded in Expectation–Confirmation Theory and the Service-Profit Chain framework, the study conceptualizes tourist satisfaction as a strategic intangible asset that contributes to financial performance through both direct and indirect mechanisms. A mixed-methods sequential explanatory design w...
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Assessment of Site Development Compliance with GBC Indonesia Greenship Criteria in a Technology Office Complex
The increasing demand for land in urban development highlights the urgent need for sustainable construction practices. While green building concepts have gained global traction, the application of appropriate site development remains understudied in the Indonesian context. This study evaluates the implementation of the Appropriate Site Development (ASD) category based on the Greenship New Building v1.2 rating tool developed by the Green Building Council Indonesia (GBCI), using the Techno Buildin...
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