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Waterbirds as bioindicators of ecosystem health: seasonal dynamics in Tondano Lake, Indonesia
Waterbird diversity in Tondano Lake reflects ecological degradation caused by water-hyacinth invasion and sedimentation. This study analyzed the diversity and abundance of waterbirds in Tondano Lake, Indonesia, across wet (February-May) and dry (June-October) seasons in 2025 to assess their role as bioindicators of ecosystem health. Multi-season observations were carried out monthly along a 6.5 km transect at ten strategic points. Eight waterbird species from three families were recorded, with E...
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Assessment of genetic diversity in EMS-induced porang (Amorphophallus muelleri Blume) revealed by RAPD markers
Haritzah, Rendha Maulana
; Radita, Novia Fitri
; Ramadanti, Isma Salsabila
; Azzahro, Yusri Despa
; Wahyudi, Didik
; Resmisari, Ruri Siti
; Suyono
Increasing genetic diversity of porang through mutation is urgently needed since porang has low genetic diveristy. Therefore, this study aims to increase the genetic diversity of porang using ethyl methanesulfonate (EMS) and analyze the genetic diversity of porang using RAPD markers. Porang explants were subjected to in vitro EMS induction at various concentrations (control, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and evaluated after 35 days. DNA amplification was carried out using 20 RAPD primers. Ge...
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Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain
Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control...
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Utilization of cassava peel waste (Manihot esculenta) as substrate in the production of cellulase enzymes by Aspergillus niger
Cellulase enzymes is an enzyme that can hydrolyze cellulose into smaller sugar components. Cellulase enzymes are produced due to high demand, with 29.17% in the animal feed industry, 14.67% in the textile industry, and 26.17% in the paper industry. One method of producing cellulase enzymes is by utilizing the cellulolytic microorganism Aspergillus niger. This study aims to optimize cellulase enzyme production by Aspergillus niger through liquid fermentation on cassava peel substrate (Manihot esc...
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Isolation and screening of halotolerant protease-producing bacteria from Lampung fermented shrimp paste
Protease are class of degradative enzymes that hydrolyze proteins, breaking its peptide bond, into smaller peptides and amino acids. Microbial proteases have gained increasing attention due to its productivity and utilization in various industries. Proteolytic bacteria derived from fermented shrimp paste from Lampung have great potential to meet this need. However, studies on these bacteria are still limited, so further research is needed to explore and optimally utilize their potential. This st...
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Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches
Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. an...
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A The Impact of Tourism Regulations on Tourist Visit Performance: A Comparative Analysis of Indonesia and Singapore
Tourism regulations play a critical role in shaping destination competitiveness and influencing tourist travel decisions, yet empirical evidence on their effectiveness across different institutional contexts remains limited. This study investigates the impact of tourism regulations on tourist visit performance through a comparative quantitative analysis of Indonesia and Singapore. Using panel data covering the period 2010–2023, this research employs a fixed-effects regression model to examine th...
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Lean Manufacturing through VSM and SMED for Waste Reduction in Paper-Based Packaging
This study analyzes the reasons for waste in the production processes of a paper-based food packaging company in Indonesia and proposes improvement measures to make the operations more efficient. Through the application of Value Stream Mapping (VSM) and Single-Minute Exchange of Dies (SMED), three significant waste categories were charted: product defects (24.74%), unnecessary movements (22.23%), and unnecessary inventory (13.29%). Implementation of SMED successfully reduced the time of tool cha...
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Oligonuclear Cobalt Assembly with Schiff-base Ligands
In order to develop molecular magnetic compounds, oligonuclear cobalt complexes with Schiff-base ligands, 1,3-bis(salicylideneamino)-2-propanol (H3salpr), 1,3-bis(5-chlorosalicylideneamino)-2-propanol (H3clsalpr), 1,3-bis(5-bromosalicylideneamino)-2-propanol (H3brsalpr), and 1,3-bis(5-nitrosalicylideneamino)-2-propanol (H3pnsalpr), were synthesized by reaction of cobalt(II) acetate and Schiff-base ligand in organic solvent. The molecular structures of [CoII{CoIII(µ-HL)(µ-CH3COO)(CH3COO)}2] (HL =...
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Analysis for Non-covalent Bonds in Protein Structures Using the DV-Xα Method
Halogen atoms are increasingly recognized for their ability to form non-covalent halogen bonds in protein–ligand complexesy. To systematically evaluate these interactions, we employed the DV-Xα method to calculate bond overlap populations (BOPs) in protein structures containing halogenated ligands. Using the Protein Data Bank, we identified thousands of entries with fluorine, chlorine, bromine, or iodine atoms. Structural coordinates were extracted around the chlorine atoms of the ligand and sub...
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